Construct: shRNA TRCN0000185385

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146829.1-425s1c1
DNA Barcode:
GTTCTCTGATTTCAGGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr27 (258826)
Gene Description:
olfactory receptor 27
Transcript:
RefSeq NM_146829.1 (NON-CURRENT)
Match location:
Position 425 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000185385)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCTCTGATTTCAGGAATAT
Hairpin Sequence:
5'-CCGG-GTTCTCTGATTTCAGGAATAT-CTCGAG-ATATTCCTGAAATCAGAGAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCTCTGATTTCAGGAATATCTCGAGATATTCCTGAAATCAGAGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCTCTGATTTCAGGAATATCTCGAGATATTCCTGAAATCAGAGAAC-3'

Other clones with same target sequence:

(none)