Construct: shRNA TRCN0000185403
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_001005338.1-243s1c1
- DNA Barcode:
- GCTGAATAACTTCTTAGCTAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- OR5H1 (26341)
- Gene Description:
- olfactory receptor family 5 subfamily H member 1
- Transcript:
- RefSeq NM_001005338.1 (CURRENT)
- Match location:
- Position 243 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000185403)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGAATAACTTCTTAGCTAA
- Hairpin Sequence:
-
5'-CCGG-GCTGAATAACTTCTTAGCTAA-CTCGAG-TTAGCTAAGAAGTTATTCAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGAATAACTTCTTAGCTAACTCGAGTTAGCTAAGAAGTTATTCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGAATAACTTCTTAGCTAACTCGAGTTAGCTAAGAAGTTATTCAGC-3'
Other clones with same target sequence:
(none)
