Construct: shRNA TRCN0000185408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146485.1-243s1c1
DNA Barcode:
GCTATTTAATCTCTTGGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr183 (258478)
Gene Description:
olfactory receptor 183
Transcript:
RefSeq NM_146485.1 (NON-CURRENT)
Match location:
Position 243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000185408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTATTTAATCTCTTGGACAA
Hairpin Sequence:
5'-CCGG-GCTATTTAATCTCTTGGACAA-CTCGAG-TTGTCCAAGAGATTAAATAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTATTTAATCTCTTGGACAACTCGAGTTGTCCAAGAGATTAAATAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTATTTAATCTCTTGGACAACTCGAGTTGTCCAAGAGATTAAATAGC-3'

Other clones with same target sequence:

(none)