Construct: shRNA TRCN0000185699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_088951.5-2334s1c1
DNA Barcode:
GCAGTATGTATTTCTACCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OLFM3 (118427)
Gene Description:
olfactomedin 3
Transcript:
RefSeq XM_088951.5 (NON-CURRENT)
Match location:
Position 2334 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000185699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTATGTATTTCTACCATT
Hairpin Sequence:
5'-CCGG-GCAGTATGTATTTCTACCATT-CTCGAG-AATGGTAGAAATACATACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTATGTATTTCTACCATTCTCGAGAATGGTAGAAATACATACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTATGTATTTCTACCATTCTCGAGAATGGTAGAAATACATACTGC-3'

Other clones with same target sequence:

(none)