Construct: shRNA TRCN0000185843

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019476.1-514s1c1
DNA Barcode:
GACAATGTCATCAATCACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr159 (29849)
Gene Description:
olfactory receptor 159
Transcript:
RefSeq NM_019476.1 (CURRENT)
Match location:
Position 514 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000185843)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAATGTCATCAATCACTTT
Hairpin Sequence:
5'-CCGG-GACAATGTCATCAATCACTTT-CTCGAG-AAAGTGATTGATGACATTGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAATGTCATCAATCACTTTCTCGAGAAAGTGATTGATGACATTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAATGTCATCAATCACTTTCTCGAGAAAGTGATTGATGACATTGTC-3'

Other clones with same target sequence:

(none)