Construct: shRNA TRCN0000185847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146369.1-598s1c1
DNA Barcode:
GCAGCTTGTGTGTTCATATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr434 (258366)
Gene Description:
olfactory receptor 434
Transcript:
RefSeq NM_146369.1 (CURRENT)
Match location:
Position 598 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000185847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCTTGTGTGTTCATATTA
Hairpin Sequence:
5'-CCGG-GCAGCTTGTGTGTTCATATTA-CTCGAG-TAATATGAACACACAAGCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCTTGTGTGTTCATATTACTCGAGTAATATGAACACACAAGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCTTGTGTGTTCATATTACTCGAGTAATATGAACACACAAGCTGC-3'

Other clones with same target sequence:

(none)