Construct: shRNA TRCN0000186057

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146682.1-804s1c1
DNA Barcode:
CTACTCATTTAGCAGAGACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr76 (258677)
Gene Description:
olfactory receptor 76
Transcript:
RefSeq NM_146682.1 (CURRENT)
Match location:
Position 804 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186057)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTCATTTAGCAGAGACAA
Hairpin Sequence:
5'-CCGG-CTACTCATTTAGCAGAGACAA-CTCGAG-TTGTCTCTGCTAAATGAGTAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTCATTTAGCAGAGACAACTCGAGTTGTCTCTGCTAAATGAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTCATTTAGCAGAGACAACTCGAGTTGTCTCTGCTAAATGAGTAG-3'

Other clones with same target sequence:

(none)