Construct: shRNA TRCN0000186182

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146655.1-384s1c1
DNA Barcode:
CCTTCACTACACTGTCATCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr441 (258649)
Gene Description:
olfactory receptor 441
Transcript:
RefSeq NM_146655.1 (CURRENT)
Match location:
Position 384 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186182)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCACTACACTGTCATCAT
Hairpin Sequence:
5'-CCGG-CCTTCACTACACTGTCATCAT-CTCGAG-ATGATGACAGTGTAGTGAAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCACTACACTGTCATCATCTCGAGATGATGACAGTGTAGTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCACTACACTGTCATCATCTCGAGATGATGACAGTGTAGTGAAGG-3'

Other clones with same target sequence:

(none)