Construct: shRNA TRCN0000186231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_374422.1-769s1c1
DNA Barcode:
CTTGTTCTTCAGATGTTGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPY19L1 (23333)
Gene Description:
dpy-19 like C-mannosyltransferase 1
Transcript:
RefSeq XM_374422.1 (NON-CURRENT)
Match location:
Position 769 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTTCTTCAGATGTTGCTA
Hairpin Sequence:
5'-CCGG-CTTGTTCTTCAGATGTTGCTA-CTCGAG-TAGCAACATCTGAAGAACAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTTCTTCAGATGTTGCTACTCGAGTAGCAACATCTGAAGAACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTTCTTCAGATGTTGCTACTCGAGTAGCAACATCTGAAGAACAAG-3'

Other clones with same target sequence:

(none)