Construct: shRNA TRCN0000186276

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291544.2-223s1c1
DNA Barcode:
CACCAGGAGAAATTTGATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC343384 (343384)
Gene Description:
peptidyl-prolyl cis-trans isomerase A-like
Transcript:
RefSeq XM_291544.2 (NON-CURRENT)
Match location:
Position 223 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186276)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCAGGAGAAATTTGATGAT
Hairpin Sequence:
5'-CCGG-CACCAGGAGAAATTTGATGAT-CTCGAG-ATCATCAAATTTCTCCTGGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCAGGAGAAATTTGATGATCTCGAGATCATCAAATTTCTCCTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCAGGAGAAATTTGATGATCTCGAGATCATCAAATTTCTCCTGGTG-3'

Other clones with same target sequence:

(none)