Construct: shRNA TRCN0000186548
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_090203.1-78s1c1
- DNA Barcode:
- GCTCTGTGCTACAATTACAAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- OR2AG1 (144125)
- Gene Description:
- olfactory receptor family 2 subfamily AG member 1 (gene/pseudogene)
- Transcript:
- RefSeq XM_090203.1 (NON-CURRENT)
- Match location:
- Position 78 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000186548)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTCTGTGCTACAATTACAAT
- Hairpin Sequence:
-
5'-CCGG-GCTCTGTGCTACAATTACAAT-CTCGAG-ATTGTAATTGTAGCACAGAGC-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTCTGTGCTACAATTACAATCTCGAGATTGTAATTGTAGCACAGAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTCTGTGCTACAATTACAATCTCGAGATTGTAATTGTAGCACAGAGC-3'
Other clones with same target sequence:
(none)
