Construct: shRNA TRCN0000186551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_497547.1-68s1c1
DNA Barcode:
GCAGAGAAGAACATAAGAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HMGB3P27 (441795)
Gene Description:
high mobility group box 3 pseudogene 27
Transcript:
RefSeq XM_497547.1 (NON-CURRENT)
Match location:
Position 68 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGAAGAACATAAGAAGA
Hairpin Sequence:
5'-CCGG-GCAGAGAAGAACATAAGAAGA-CTCGAG-TCTTCTTATGTTCTTCTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGAAGAACATAAGAAGACTCGAGTCTTCTTATGTTCTTCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGAAGAACATAAGAAGACTCGAGTCTTCTTATGTTCTTCTCTGC-3'

Other clones with same target sequence:

(none)