Construct: shRNA TRCN0000186862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_134104.6-388s1c1
DNA Barcode:
GCTGTACATAAGTACACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC627165 (627165)
Gene Description:
similar to U6 snRNA-associated Sm-like protein LSm6
Transcript:
RefSeq XM_134104.6 (NON-CURRENT)
Match location:
Position 388 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTACATAAGTACACAGAA
Hairpin Sequence:
5'-CCGG-GCTGTACATAAGTACACAGAA-CTCGAG-TTCTGTGTACTTATGTACAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTACATAAGTACACAGAACTCGAGTTCTGTGTACTTATGTACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTACATAAGTACACAGAACTCGAGTTCTGTGTACTTATGTACAGC-3'

Other clones with same target sequence:

(none)