Construct: shRNA TRCN0000186898

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_126150.2-2184s1c1
DNA Barcode:
CCTACTATTCATACAAGCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbtb4 (75580)
Gene Description:
zinc finger and BTB domain containing 4
Transcript:
RefSeq XM_126150.2 (NON-CURRENT)
Match location:
Position 2184 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186898)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACTATTCATACAAGCCTA
Hairpin Sequence:
5'-CCGG-CCTACTATTCATACAAGCCTA-CTCGAG-TAGGCTTGTATGAATAGTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACTATTCATACAAGCCTACTCGAGTAGGCTTGTATGAATAGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACTATTCATACAAGCCTACTCGAGTAGGCTTGTATGAATAGTAGG-3'

Other clones with same target sequence:

(none)