Construct: shRNA TRCN0000186901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_371606.2-889s1c1
DNA Barcode:
CACGCCAATAATTAACCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR6B2 (389090)
Gene Description:
olfactory receptor family 6 subfamily B member 2
Transcript:
RefSeq XM_371606.2 (NON-CURRENT)
Match location:
Position 889 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGCCAATAATTAACCCTTT
Hairpin Sequence:
5'-CCGG-CACGCCAATAATTAACCCTTT-CTCGAG-AAAGGGTTAATTATTGGCGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGCCAATAATTAACCCTTTCTCGAGAAAGGGTTAATTATTGGCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGCCAATAATTAACCCTTTCTCGAGAAAGGGTTAATTATTGGCGTG-3'

Other clones with same target sequence:

(none)