Construct: shRNA TRCN0000186940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_488248.1-159s1c1
DNA Barcode:
GACTGTGGACAATTTCAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC382716 (382716)
Gene Description:
similar to Hippocalcin-like protein 1 (Visinin-like protein 3) (VILIP-3) (Neural visinin-like protein 3) (NVL-3) (NVP-3)
Transcript:
RefSeq XM_488248.1 (NON-CURRENT)
Match location:
Position 159 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000186940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGTGGACAATTTCAAGAT
Hairpin Sequence:
5'-CCGG-GACTGTGGACAATTTCAAGAT-CTCGAG-ATCTTGAAATTGTCCACAGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGTGGACAATTTCAAGATCTCGAGATCTTGAAATTGTCCACAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGTGGACAATTTCAAGATCTCGAGATCTTGAAATTGTCCACAGTC-3'

Other clones with same target sequence:

(none)