Construct: shRNA TRCN0000187284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001015038.1-284s1c1
DNA Barcode:
CTTAAGATAGAGGATGAGCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAGE2B (389860)
Gene Description:
PAGE family member 2B
Transcript:
RefSeq NM_001015038.1 (NON-CURRENT)
Match location:
Position 284 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTAAGATAGAGGATGAGCCT
Hairpin Sequence:
5'-CCGG-CTTAAGATAGAGGATGAGCCT-CTCGAG-AGGCTCATCCTCTATCTTAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTAAGATAGAGGATGAGCCTCTCGAGAGGCTCATCCTCTATCTTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTAAGATAGAGGATGAGCCTCTCGAGAGGCTCATCCTCTATCTTAAG-3'

Other clones with same target sequence:

(none)