Construct: shRNA TRCN0000187316

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355972.1-752s1c1
DNA Barcode:
CCAAAGCATGTGAGGACCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381948 (381948)
Gene Description:
similar to PYRIN-containing APAF1-like protein 7
Transcript:
RefSeq XM_355972.1 (NON-CURRENT)
Match location:
Position 752 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187316)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAAGCATGTGAGGACCTTT
Hairpin Sequence:
5'-CCGG-CCAAAGCATGTGAGGACCTTT-CTCGAG-AAAGGTCCTCACATGCTTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAAGCATGTGAGGACCTTTCTCGAGAAAGGTCCTCACATGCTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAAGCATGTGAGGACCTTTCTCGAGAAAGGTCCTCACATGCTTTGG-3'

Other clones with same target sequence:

(none)