Construct: shRNA TRCN0000187345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_166777.1-348s1c1
DNA Barcode:
CATGTCCTATGATCGCTACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR10G9 (219870)
Gene Description:
olfactory receptor family 10 subfamily G member 9
Transcript:
RefSeq XM_166777.1 (NON-CURRENT)
Match location:
Position 348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGTCCTATGATCGCTACTT
Hairpin Sequence:
5'-CCGG-CATGTCCTATGATCGCTACTT-CTCGAG-AAGTAGCGATCATAGGACATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGTCCTATGATCGCTACTTCTCGAGAAGTAGCGATCATAGGACATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGTCCTATGATCGCTACTTCTCGAGAAGTAGCGATCATAGGACATG-3'

Other clones with same target sequence:

(none)