Construct: shRNA TRCN0000187421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_291862.3-594s1c1
DNA Barcode:
CCACTTCAATGGCATTGTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR5AP2 (338675)
Gene Description:
olfactory receptor family 5 subfamily AP member 2
Transcript:
RefSeq XM_291862.3 (NON-CURRENT)
Match location:
Position 594 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTTCAATGGCATTGTGAT
Hairpin Sequence:
5'-CCGG-CCACTTCAATGGCATTGTGAT-CTCGAG-ATCACAATGCCATTGAAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTTCAATGGCATTGTGATCTCGAGATCACAATGCCATTGAAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTTCAATGGCATTGTGATCTCGAGATCACAATGCCATTGAAGTGG-3'

Other clones with same target sequence:

(none)