Construct: shRNA TRCN0000187699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001011860.1-381s1c1
DNA Barcode:
GCCTCTTCACTACTCTTCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr1222 (258177)
Gene Description:
olfactory receptor 1222
Transcript:
RefSeq NM_001011860.1 (CURRENT)
Match location:
Position 381 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTTCACTACTCTTCCAT
Hairpin Sequence:
5'-CCGG-GCCTCTTCACTACTCTTCCAT-CTCGAG-ATGGAAGAGTAGTGAAGAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTTCACTACTCTTCCATCTCGAGATGGAAGAGTAGTGAAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTTCACTACTCTTCCATCTCGAGATGGAAGAGTAGTGAAGAGGC-3'

Other clones with same target sequence:

(none)