Construct: shRNA TRCN0000187941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_132038.3-2094s1c1
DNA Barcode:
GCTTCAGAATGTGACTCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stim2 (116873)
Gene Description:
stromal interaction molecule 2
Transcript:
RefSeq XM_132038.3 (NON-CURRENT)
Match location:
Position 2094 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCAGAATGTGACTCCTTA
Hairpin Sequence:
5'-CCGG-GCTTCAGAATGTGACTCCTTA-CTCGAG-TAAGGAGTCACATTCTGAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCAGAATGTGACTCCTTACTCGAGTAAGGAGTCACATTCTGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCAGAATGTGACTCCTTACTCGAGTAAGGAGTCACATTCTGAAGC-3'

Other clones with same target sequence:

(none)