Construct: shRNA TRCN0000187988

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001005504.1-255s1c1
DNA Barcode:
GTTCAGAAAGCGCAAAGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR4F21 (441308)
Gene Description:
olfactory receptor family 4 subfamily F member 21
Transcript:
RefSeq NM_001005504.1 (CURRENT)
Match location:
Position 255 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000187988)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCAGAAAGCGCAAAGTCAT
Hairpin Sequence:
5'-CCGG-GTTCAGAAAGCGCAAAGTCAT-CTCGAG-ATGACTTTGCGCTTTCTGAAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCAGAAAGCGCAAAGTCATCTCGAGATGACTTTGCGCTTTCTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCAGAAAGCGCAAAGTCATCTCGAGATGACTTTGCGCTTTCTGAAC-3'

Other clones with same target sequence:

(none)