Construct: shRNA TRCN0000188190

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_497480.1-124s1c1
DNA Barcode:
CAACTCTGCTTGACCATCTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC441742 (441742)
Gene Description:
similar to Gamma-aminobutyric-acid receptor alpha-5 subunit precursor (GABA(A) receptor)
Transcript:
RefSeq XM_497480.1 (NON-CURRENT)
Match location:
Position 124 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000188190)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTCTGCTTGACCATCTCT
Hairpin Sequence:
5'-CCGG-CAACTCTGCTTGACCATCTCT-CTCGAG-AGAGATGGTCAAGCAGAGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTCTGCTTGACCATCTCTCTCGAGAGAGATGGTCAAGCAGAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTCTGCTTGACCATCTCTCTCGAGAGAGATGGTCAAGCAGAGTTG-3'

Other clones with same target sequence:

(none)