Construct: shRNA TRCN0000188235

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058164.1-889s1c1
DNA Barcode:
CGTGGTGGTCAAATACCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OLFM2 (93145)
Gene Description:
olfactomedin 2
Transcript:
RefSeq NM_058164.1 (NON-CURRENT)
Match location:
Position 889 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000188235)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGTGGTCAAATACCACTT
Hairpin Sequence:
5'-CCGG-CGTGGTGGTCAAATACCACTT-CTCGAG-AAGTGGTATTTGACCACCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGTGGTCAAATACCACTTCTCGAGAAGTGGTATTTGACCACCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGTGGTCAAATACCACTTCTCGAGAAGTGGTATTTGACCACCACG-3'

Other clones with same target sequence:

(none)