Construct: shRNA TRCN0000188297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146280.1-170s1c1
DNA Barcode:
CGCCCATGTACTTCTTCCTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr281 (258277)
Gene Description:
olfactory receptor 281
Transcript:
RefSeq NM_146280.1 (CURRENT)
Match location:
Position 170 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000188297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCCATGTACTTCTTCCTAA
Hairpin Sequence:
5'-CCGG-CGCCCATGTACTTCTTCCTAA-CTCGAG-TTAGGAAGAAGTACATGGGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCCATGTACTTCTTCCTAACTCGAGTTAGGAAGAAGTACATGGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCCATGTACTTCTTCCTAACTCGAGTTAGGAAGAAGTACATGGGCG-3'

Other clones with same target sequence:

(none)