Construct: shRNA TRCN0000188307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_137040.1-576s1c1
DNA Barcode:
CCTCAGAAGCTCAGTGCTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sult3a2 (215895)
Gene Description:
sulfotransferase family 3A, member 2
Transcript:
RefSeq XM_137040.1 (NON-CURRENT)
Match location:
Position 576 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000188307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAGAAGCTCAGTGCTTAA
Hairpin Sequence:
5'-CCGG-CCTCAGAAGCTCAGTGCTTAA-CTCGAG-TTAAGCACTGAGCTTCTGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAGAAGCTCAGTGCTTAACTCGAGTTAAGCACTGAGCTTCTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAGAAGCTCAGTGCTTAACTCGAGTTAAGCACTGAGCTTCTGAGG-3'

Other clones with same target sequence:

(none)