Construct: shRNA TRCN0000188637

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146926.1-310s1c1
DNA Barcode:
GCAGTGACATTTGGGACAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Olfr477 (258928)
Gene Description:
olfactory receptor 477
Transcript:
RefSeq NM_146926.1 (CURRENT)
Match location:
Position 310 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000188637)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGACATTTGGGACAGTT
Hairpin Sequence:
5'-CCGG-GCAGTGACATTTGGGACAGTT-CTCGAG-AACTGTCCCAAATGTCACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGACATTTGGGACAGTTCTCGAGAACTGTCCCAAATGTCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGACATTTGGGACAGTTCTCGAGAACTGTCCCAAATGTCACTGC-3'

Other clones with same target sequence:

(none)