Construct: shRNA TRCN0000188775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013675.1-45s1c1
DNA Barcode:
GCAGAAAGAAGAGGCTATGGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRC74B (400891)
Gene Description:
leucine rich repeat containing 74B
Transcript:
RefSeq NM_001013675.1 (NON-CURRENT)
Match location:
Position 45 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000188775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAAAGAAGAGGCTATGGT
Hairpin Sequence:
5'-CCGG-GCAGAAAGAAGAGGCTATGGT-CTCGAG-ACCATAGCCTCTTCTTTCTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAAAGAAGAGGCTATGGTCTCGAGACCATAGCCTCTTCTTTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAAAGAAGAGGCTATGGTCTCGAGACCATAGCCTCTTCTTTCTGC-3'

Other clones with same target sequence:

(none)