Construct: shRNA TRCN0000188863

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_376619.1-196s1c1
DNA Barcode:
GCACAGCTCCATCGTTACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC401357 (401357)
Gene Description:
uncharacterized LOC401357
Transcript:
RefSeq XM_376619.1 (NON-CURRENT)
Match location:
Position 196 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000188863)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAGCTCCATCGTTACAAT
Hairpin Sequence:
5'-CCGG-GCACAGCTCCATCGTTACAAT-CTCGAG-ATTGTAACGATGGAGCTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAGCTCCATCGTTACAATCTCGAGATTGTAACGATGGAGCTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAGCTCCATCGTTACAATCTCGAGATTGTAACGATGGAGCTGTGC-3'

Other clones with same target sequence:

(none)