Construct: shRNA TRCN0000189223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_379214.2-1033s1c1
DNA Barcode:
GCATGGGCATTTCTTCCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H1FX-AS1 (339942)
Gene Description:
H1FX antisense RNA 1
Transcript:
RefSeq XM_379214.2 (NON-CURRENT)
Match location:
Position 1033 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGGCATTTCTTCCTGTT
Hairpin Sequence:
5'-CCGG-GCATGGGCATTTCTTCCTGTT-CTCGAG-AACAGGAAGAAATGCCCATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGGCATTTCTTCCTGTTCTCGAGAACAGGAAGAAATGCCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGGCATTTCTTCCTGTTCTCGAGAACAGGAAGAAATGCCCATGC-3'

Other clones with same target sequence:

(none)