Construct: shRNA TRCN0000189478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028354.1-771s1c1
DNA Barcode:
CGAAGCCCTATGCGAACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tdp1 (104884)
Gene Description:
tyrosyl-DNA phosphodiesterase 1
Transcript:
RefSeq NM_028354.1 (NON-CURRENT)
Match location:
Position 771 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGCCCTATGCGAACATTT
Hairpin Sequence:
5'-CCGG-CGAAGCCCTATGCGAACATTT-CTCGAG-AAATGTTCGCATAGGGCTTCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGCCCTATGCGAACATTTCTCGAGAAATGTTCGCATAGGGCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGCCCTATGCGAACATTTCTCGAGAAATGTTCGCATAGGGCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000257079