Construct: shRNA TRCN0000189540

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026866.1-3748s1c1
DNA Barcode:
CCAGATCCCTTTCCCTACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Disp1 (68897)
Gene Description:
dispatched RND transporter family member 1
Transcript:
RefSeq NM_026866.1 (NON-CURRENT)
Match location:
Position 3748 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189540)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATCCCTTTCCCTACAAA
Hairpin Sequence:
5'-CCGG-CCAGATCCCTTTCCCTACAAA-CTCGAG-TTTGTAGGGAAAGGGATCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATCCCTTTCCCTACAAACTCGAGTTTGTAGGGAAAGGGATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATCCCTTTCCCTACAAACTCGAGTTTGTAGGGAAAGGGATCTGG-3'

Other clones with same target sequence:

(none)