Construct: shRNA TRCN0000189650

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026892.1-437s1c1
DNA Barcode:
CAAAGGAAGAACATCTGCCAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif1b (68969)
Gene Description:
eukaryotic translation initiation factor 1B
Transcript:
RefSeq NM_026892.1 (NON-CURRENT)
Match location:
Position 437 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189650)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGGAAGAACATCTGCCAG
Hairpin Sequence:
5'-CCGG-CAAAGGAAGAACATCTGCCAG-CTCGAG-CTGGCAGATGTTCTTCCTTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGGAAGAACATCTGCCAGCTCGAGCTGGCAGATGTTCTTCCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGGAAGAACATCTGCCAGCTCGAGCTGGCAGATGTTCTTCCTTTG-3'

Other clones with same target sequence:

(none)