Construct: shRNA TRCN0000189653

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145562.1-775s1c1
DNA Barcode:
CAACAGAAGAGCACAGCTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Parm1 (231440)
Gene Description:
prostate androgen-regulated mucin-like protein 1
Transcript:
RefSeq NM_145562.1 (NON-CURRENT)
Match location:
Position 775 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189653)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACAGAAGAGCACAGCTCTA
Hairpin Sequence:
5'-CCGG-CAACAGAAGAGCACAGCTCTA-CTCGAG-TAGAGCTGTGCTCTTCTGTTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACAGAAGAGCACAGCTCTACTCGAGTAGAGCTGTGCTCTTCTGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACAGAAGAGCACAGCTCTACTCGAGTAGAGCTGTGCTCTTCTGTTG-3'

Other clones with same target sequence:

  1. TRCN0000279119