Construct: shRNA TRCN0000189653
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_145562.1-775s1c1
- DNA Barcode:
- CAACAGAAGAGCACAGCTCTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Parm1 (231440)
- Gene Description:
- prostate androgen-regulated mucin-like protein 1
- Transcript:
- RefSeq NM_145562.1 (NON-CURRENT)
- Match location:
- Position 775 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000189653)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CAACAGAAGAGCACAGCTCTA
- Hairpin Sequence:
-
5'-CCGG-CAACAGAAGAGCACAGCTCTA-CTCGAG-TAGAGCTGTGCTCTTCTGTTG-TTTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCAACAGAAGAGCACAGCTCTACTCGAGTAGAGCTGTGCTCTTCTGTTGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACAACAGAAGAGCACAGCTCTACTCGAGTAGAGCTGTGCTCTTCTGTTG-3'
Other clones with same target sequence:
-
TRCN0000279119
