Construct: shRNA TRCN0000189735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178705.4-1395s1c1
DNA Barcode:
GCCATCAAATCCAACCCAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Luzp2 (233271)
Gene Description:
leucine zipper protein 2
Transcript:
RefSeq NM_178705.4 (NON-CURRENT)
Match location:
Position 1395 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCAAATCCAACCCAGAT
Hairpin Sequence:
5'-CCGG-GCCATCAAATCCAACCCAGAT-CTCGAG-ATCTGGGTTGGATTTGATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCAAATCCAACCCAGATCTCGAGATCTGGGTTGGATTTGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCAAATCCAACCCAGATCTCGAGATCTGGGTTGGATTTGATGGC-3'

Other clones with same target sequence:

(none)