Construct: shRNA TRCN0000189789

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173778.2-297s1c1
DNA Barcode:
GCACAAGAAAGCCCTAGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh2d7 (244885)
Gene Description:
SH2 domain containing 7
Transcript:
RefSeq NM_173778.2 (NON-CURRENT)
Match location:
Position 297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189789)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAGAAAGCCCTAGACAT
Hairpin Sequence:
5'-CCGG-GCACAAGAAAGCCCTAGACAT-CTCGAG-ATGTCTAGGGCTTTCTTGTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAGAAAGCCCTAGACATCTCGAGATGTCTAGGGCTTTCTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAGAAAGCCCTAGACATCTCGAGATGTCTAGGGCTTTCTTGTGC-3'

Other clones with same target sequence:

(none)