Construct: shRNA TRCN0000189925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033312.1-328s1c1
DNA Barcode:
GAAGGTGAAGCAACTGGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxl18 (231863)
Gene Description:
F-box and leucine-rich repeat protein 18
Transcript:
RefSeq NM_001033312.1 (NON-CURRENT)
Match location:
Position 328 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGGTGAAGCAACTGGTGAA
Hairpin Sequence:
5'-CCGG-GAAGGTGAAGCAACTGGTGAA-CTCGAG-TTCACCAGTTGCTTCACCTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGGTGAAGCAACTGGTGAACTCGAGTTCACCAGTTGCTTCACCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGGTGAAGCAACTGGTGAACTCGAGTTCACCAGTTGCTTCACCTTC-3'

Other clones with same target sequence:

(none)