Construct: shRNA TRCN0000189941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175016.1-671s1c1
DNA Barcode:
GCGCTTGCAGAGACTTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Alkbh2 (231642)
Gene Description:
alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase
Transcript:
RefSeq NM_175016.1 (NON-CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTTGCAGAGACTTCATTT
Hairpin Sequence:
5'-CCGG-GCGCTTGCAGAGACTTCATTT-CTCGAG-AAATGAAGTCTCTGCAAGCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTTGCAGAGACTTCATTTCTCGAGAAATGAAGTCTCTGCAAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTTGCAGAGACTTCATTTCTCGAGAAATGAAGTCTCTGCAAGCGC-3'

Other clones with same target sequence:

(none)