Construct: shRNA TRCN0000189953

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027238.1-179s1c1
DNA Barcode:
GCGGCTTTCACTTTGTACCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ttc39b (69863)
Gene Description:
tetratricopeptide repeat domain 39B
Transcript:
RefSeq NM_027238.1 (NON-CURRENT)
Match location:
Position 179 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189953)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTTTCACTTTGTACCAT
Hairpin Sequence:
5'-CCGG-GCGGCTTTCACTTTGTACCAT-CTCGAG-ATGGTACAAAGTGAAAGCCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTTTCACTTTGTACCATCTCGAGATGGTACAAAGTGAAAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTTTCACTTTGTACCATCTCGAGATGGTACAAAGTGAAAGCCGC-3'

Other clones with same target sequence:

(none)