Construct: shRNA TRCN0000189992

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015798.1-272s1c1
DNA Barcode:
GCCATCGGAAATCTTGGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxo15 (50764)
Gene Description:
F-box protein 15
Transcript:
RefSeq NM_015798.1 (NON-CURRENT)
Match location:
Position 272 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000189992)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATCGGAAATCTTGGTGAA
Hairpin Sequence:
5'-CCGG-GCCATCGGAAATCTTGGTGAA-CTCGAG-TTCACCAAGATTTCCGATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATCGGAAATCTTGGTGAACTCGAGTTCACCAAGATTTCCGATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATCGGAAATCTTGGTGAACTCGAGTTCACCAAGATTTCCGATGGC-3'

Other clones with same target sequence:

(none)