Construct: shRNA TRCN0000190003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178664.2-1010s1c1
DNA Barcode:
GCTACGAAGACTCACAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B3gntl1 (210004)
Gene Description:
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase-like 1
Transcript:
RefSeq NM_178664.2 (NON-CURRENT)
Match location:
Position 1010 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTACGAAGACTCACAGGAAA
Hairpin Sequence:
5'-CCGG-GCTACGAAGACTCACAGGAAA-CTCGAG-TTTCCTGTGAGTCTTCGTAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTACGAAGACTCACAGGAAACTCGAGTTTCCTGTGAGTCTTCGTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTACGAAGACTCACAGGAAACTCGAGTTTCCTGTGAGTCTTCGTAGC-3'

Other clones with same target sequence:

(none)