Construct: shRNA TRCN0000190061

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133924.1-432s1c1
DNA Barcode:
GCCAATGTTGTCAAAGACCCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snx21 (101113)
Gene Description:
sorting nexin family member 21
Transcript:
RefSeq NM_133924.1 (NON-CURRENT)
Match location:
Position 432 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190061)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATGTTGTCAAAGACCCT
Hairpin Sequence:
5'-CCGG-GCCAATGTTGTCAAAGACCCT-CTCGAG-AGGGTCTTTGACAACATTGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATGTTGTCAAAGACCCTCTCGAGAGGGTCTTTGACAACATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATGTTGTCAAAGACCCTCTCGAGAGGGTCTTTGACAACATTGGC-3'

Other clones with same target sequence:

  1. TRCN0000363920