Construct: shRNA TRCN0000190083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026641.1-2224s1c1
DNA Barcode:
GCTCGCTTATCGTCAGAAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ift80 (68259)
Gene Description:
intraflagellar transport 80
Transcript:
RefSeq NM_026641.1 (NON-CURRENT)
Match location:
Position 2224 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGCTTATCGTCAGAAGTT
Hairpin Sequence:
5'-CCGG-GCTCGCTTATCGTCAGAAGTT-CTCGAG-AACTTCTGACGATAAGCGAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGCTTATCGTCAGAAGTTCTCGAGAACTTCTGACGATAAGCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGCTTATCGTCAGAAGTTCTCGAGAACTTCTGACGATAAGCGAGC-3'

Other clones with same target sequence:

  1. TRCN0000345149