Construct: shRNA TRCN0000190194

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139140.1-1324s1c1
DNA Barcode:
CACTTTGTCAGTGAGCGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spats2 (72572)
Gene Description:
spermatogenesis associated, serine-rich 2
Transcript:
RefSeq NM_139140.1 (CURRENT)
Match location:
Position 1324 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190194)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACTTTGTCAGTGAGCGGAAA
Hairpin Sequence:
5'-CCGG-CACTTTGTCAGTGAGCGGAAA-CTCGAG-TTTCCGCTCACTGACAAAGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACTTTGTCAGTGAGCGGAAACTCGAGTTTCCGCTCACTGACAAAGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACTTTGTCAGTGAGCGGAAACTCGAGTTTCCGCTCACTGACAAAGTG-3'

Other clones with same target sequence:

(none)