Construct: shRNA TRCN0000190308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178797.2-1602s1c1
DNA Barcode:
CGGAACGTCTGGTTCTTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Far2 (330450)
Gene Description:
fatty acyl CoA reductase 2
Transcript:
RefSeq NM_178797.2 (NON-CURRENT)
Match location:
Position 1602 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAACGTCTGGTTCTTCATT
Hairpin Sequence:
5'-CCGG-CGGAACGTCTGGTTCTTCATT-CTCGAG-AATGAAGAACCAGACGTTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAACGTCTGGTTCTTCATTCTCGAGAATGAAGAACCAGACGTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAACGTCTGGTTCTTCATTCTCGAGAATGAAGAACCAGACGTTCCG-3'

Other clones with same target sequence:

(none)