Construct: shRNA TRCN0000190324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173425.1-1557s1c1
DNA Barcode:
CCTAGTCAAAGGGACTTTGGT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam124b (241128)
Gene Description:
family with sequence similarity 124, member B
Transcript:
RefSeq NM_173425.1 (NON-CURRENT)
Match location:
Position 1557 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGTCAAAGGGACTTTGGT
Hairpin Sequence:
5'-CCGG-CCTAGTCAAAGGGACTTTGGT-CTCGAG-ACCAAAGTCCCTTTGACTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGTCAAAGGGACTTTGGTCTCGAGACCAAAGTCCCTTTGACTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGTCAAAGGGACTTTGGTCTCGAGACCAAAGTCCCTTTGACTAGG-3'

Other clones with same target sequence:

(none)