Construct: shRNA TRCN0000190511

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178599.2-1635s1c1
DNA Barcode:
GCTGCGACATAGCAGTGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Commd8 (27784)
Gene Description:
COMM domain containing 8
Transcript:
RefSeq NM_178599.2 (NON-CURRENT)
Match location:
Position 1635 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190511)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCGACATAGCAGTGATTA
Hairpin Sequence:
5'-CCGG-GCTGCGACATAGCAGTGATTA-CTCGAG-TAATCACTGCTATGTCGCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCGACATAGCAGTGATTACTCGAGTAATCACTGCTATGTCGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCGACATAGCAGTGATTACTCGAGTAATCACTGCTATGTCGCAGC-3'

Other clones with same target sequence:

(none)