Construct: shRNA TRCN0000190515

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199306.1-954s1c1
DNA Barcode:
GCAGTATTATGTCGCAGGTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdtc1 (230796)
Gene Description:
WD and tetratricopeptide repeats 1
Transcript:
RefSeq NM_199306.1 (NON-CURRENT)
Match location:
Position 954 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190515)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTATTATGTCGCAGGTCA
Hairpin Sequence:
5'-CCGG-GCAGTATTATGTCGCAGGTCA-CTCGAG-TGACCTGCGACATAATACTGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTATTATGTCGCAGGTCACTCGAGTGACCTGCGACATAATACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTATTATGTCGCAGGTCACTCGAGTGACCTGCGACATAATACTGC-3'

Other clones with same target sequence:

(none)