Construct: shRNA TRCN0000190517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033797.1-492s1c1
DNA Barcode:
GAGGAGGAAGAAGAAGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
G430095P16Rik (619292)
Gene Description:
RIKEN cDNA G430095P16 gene
Transcript:
RefSeq NM_001033797.1 (NON-CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000190517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGGAAGAAGAAGAAGAA
Hairpin Sequence:
5'-CCGG-GAGGAGGAAGAAGAAGAAGAA-CTCGAG-TTCTTCTTCTTCTTCCTCCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGGAAGAAGAAGAAGAACTCGAGTTCTTCTTCTTCTTCCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGGAAGAAGAAGAAGAACTCGAGTTCTTCTTCTTCTTCCTCCTC-3'

Other clones with same target sequence:

(none)